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NanoDrop Microvolume Spectrophotometers

NanoDrop Microvolume Spectrophotometers

Model NO. Nanobio-500

Delivery FOB

minimum order 1 unit

Country of Origin Made in China

NanoBio 500 is a full-wavelength (190 ~ 850 nm) ultra-micro spectrophotometer developed by Optosky. It is based on Optosky's more than 20 years of experience in the development of spectral instruments. The high-stability pulsed xenon lamp (or long-life LED) is used as the light source, which can detect nucleic acid and protein quickly and accurately.

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Description

Nanobio-500 is a improved micro-spectrophotometer based on Nanobio-300 with full wavelength (200-800 nm). It added a function of fluorescence and without requiring a computer. With a sample size of only 0.5μL.to 2μL, the sample concentration can be rapidly and accurately detected.

The cuvette mode can be used to detect the concentration of culture media such as bacteria.The newly added fluorescence detection function, combined with the fluorescence quantitative analysis kit, can accurately quantify the concentration of DNA,RNA and protein through the specific combination of the fluorescent dye and the target substance, and the minimum limit can reach 0.5 pg/μL. (ds DNA).

Model	Feature
Nanobio-500	Fluorescence detection, comparison cell mode, DNA, RNA, protein concentration detection

Features

• Patented motor lifting structure .
• Standard OD600 detection function.
• Android system, 7-inch capacitive touch screen.
• High-resolution CCD array detector,6s can complete detection and display results.
• Long life pulse xenon lamp light source.
• The detection data can be transferred to the computer through USB

Application

• Nucleic acid quantification,plant GUS reporter gene detection, apoptosis detection.
• dsDNA, ssDNA, GFP, gene detection, fluorescein detection, proteinquantification.
• Rhodamine detection,Cy-3fluorescence labeling detection, RFP gene detection,cytotoxicity detection.
• Cy-5 fluorescence labeling detection, RNA quantification.

 

Technical Parameters

              Nanobio-300                    Nanobio-200                      Nanobio-500

Wavelength range 200~800 nm 260 nm, 280 nm 200~800 nm Minimum sample size 0.5~2.0 μL 1.0~2.0 μL 0.5~2.0 μL Path length 0.2 mm 1.0 mm 0.5 mm 0.05 / 0.2 mm 1.0 mm Light source Xenon flash lamp UV LED Xenon flash lamp Detector type 2048-linear CCD array UV-silicon photocell 2048-linear CCD array Wavelength accuracy 1 nm ---- 1 nm Spectral resolution ≤ 3 nm ≤ 8 nm ≤3 nm Absorbance precision 0.003 Abs 0.005 Abs 0.003 Abs Absorbance accuracy 1 %  (7.332 Abs at 260 nm) 2 %  (7.332 Abs at 260 nm) 1 %  (7.332 Abs at 260 nm) Absorbance range 0.04~90 A 0.2~50 A 0.04~300 A Nucleic acid detection range 2~4500 ng/μL (dsDNA) 10~2500 ng/μL (dsDNA) 2~15000 ng/μL (dsDNA) Measurement time < 5 s < 6 s < 6 s Dimension (W×D×H) mm 210×268×181 208×280×186 208×320×186 Weight 2.8 kg 2.0 kg 3.6 kg Sample pedestal material Aluminum alloy and quartz fiber Aluminum alloy and quartz fiber Aluminum alloy and quartz fiber Operating voltage DC 24 V 2 A DC 24 V 2 A DC 24 V 2 A Operating power 25 W 25 W 25 W Standby power 5 W 5 W 5 W Software compatibility Android system Android system Android system

 

Application

Channel	Excitation wavelength	Application
UV channel	365±20 nm	Nucleic acid quantification, plant GUS reporter gene detection, apoptosis detection
Blue channel	460±20 nm	dsDNA, ssDNA, GFP, gene detection, fluorescein detection, protein quantification
Green channel	525±20 nm	Rhodamine detection, Cy-3 fluorescence labeling detection, RFP gene detection, cytotoxicity detection
Red channel	625±20 nm	Cy-5 fluorescence labeling detection, RNA quantification

 

Parameter

 

Fluorescence Detection Mode - Specification
Light source	LED
Dynamic range	5 orders of magnitude
Linear dynamic range	R² ≥0.995
Detector	Photodiode
Repeatability	≤1.5 %
Stability	≤1.5 %
Sensitivity	dsDNA: 0.5 pg/μL
Measurement speed	3 s (once)

Datasheet Download:

Nanobio500_Datasheet_V1.0_20230816.pdf