Raman effect is first discovered by C.V. Raman and K.S Krishan in 1928. When a sample exposes monochromatic light, the sample absorbs the light, visual portion of light gets transmitted to the sample. However, a minor part of the light is scattered by the sample in all direction. Incident light has a particular frequency, if scattering light has frequency same as incident light, the scattering is called Rayleigh scattering. It has been observed that about 1% of total scatter intensity occurs frequency different from incident frequency, this is called Raman scattering. Raman scattering can be called a two photon process, an electron has different vibrational levels, they are defined by different specific energy differences.
The generation of a Raman spectrum begins with an excitation laser, routed to the sample via a Raman probe. Scattered Raman light is collected by the probe and measured by a spectrometer with the sensitivity and Raman shift range suitable for your application. Complete the system with a sample holder that accepts cuvettes, probes or SERS substrates, plus software and laser safety glasses.At the heart of each modular Raman setup is the spectrometer. Options range from our high-performance ATP6500 Scientific-Class spectrometer to the recently introduced 1064nm NIR Raman spectrometer, a more accessible option for budget-conscious researchers and product integrators.