Application of ATR8300 Raman Microscope in Testing Amino Acids

2024-01-24

Amino acid, organic molecule, a general name for a class of organic compounds containing amino and carboxyl groups. The basic unit of biologically functional macromolecule protein is the basic substance that constitutes the protein required for animal nutrition. Amino acids can play the following roles through metabolism in the human body: ① synthesize tissue proteins; ② turn into acids, hormones, antibodies, creatine and other ammonia-containing substances; ③ turn into carbohydrates and fats; ④ oxidize into carbon dioxide, water and urea, generate energy.

Amino acids are mainly used in health and wellness foods, functional fortified foods, animal feeds, food additives, cosmetics and other industries. For example, glutamic acid is one of the basic amino acids for nitrogen metabolism in biological organisms; glycine can be used as a nutritional additive for chicken feed and can be used as a non-toxic decarburization agent in the nitrogen fertilizer industry; alanine can prevent kidney stones and assist in the metabolism of glucose, help alleviate hypoglycemia and improve body energy.

My country is a supplier of amino acid raw materials and an important demander of amino acid products. Various terminals are becoming active with the activity of some emerging markets. It can be seen that the authenticity detection of amino acids is particularly important.

Arginine

Glutamic acid

Alanine

Valine

Glycine

Figure 1: Photographs of amino acids focused under the ATR8300-785 Raman microscope lens.

Optosky ATR8300-785 Raman microscope has the advantages of achieving precise positioning and measurement of micro-area Raman spectra, fast, accurate, and non-destructive analysis of components and identification of substances. It is widely used in agriculture and food identification, nanoparticle and new materials, biological sciences, drug testing, environmental testing and other fields.

Test case

This time we used the ATR8300-785 Raman microscope to test several amino acid samples from customers. As shown in the figure below, we can see that the Raman spectrum of the amino acids is perfect and the characteristic peaks are obvious, which can effectively distinguish different amino acid types. The results show the practical application of our ATR8300-785 Raman microscope in the biomedical field.

Figure 2: Data plot of each amino acid measured by ATR8300-785 Raman microscope.

Optosky ATR8300 Raman microscope combines the advantages of Raman spectrometer and microscope, making it possible to "what you see is what you measure". By focusing the incident laser onto the sample through a microscope, various Raman spectral information such as chemical composition, crystal structure, molecular interaction, and molecular orientation of the illuminated sample microarea can be accurately obtained without interference from surrounding substances.

ATR8300 has no optical path switching moving parts. All optical components are solid-state assembly and work very stably. It perfectly solves the loss of light path during camera imaging, realizes the separation of camera imaging and Raman signal collection, and obtains the best signal strength. At the same time, ATR8300 uses high-performance Raman specially optimized for Raman microscope systems. It is industry-leading in terms of sensitivity, signal-to-noise ratio, stability, etc., providing a strong guarantee for Raman research.