RAMAN FAQ
author: Admin
2022-08-22
RAMAN FAQ
A: In-situ Raman system requires higher performance, customized probe with quantitative analysis algorithm.
Q2: How to choose Optosky's Raman spectrometer series models?
A: Firstly, ATR represents Optosky's Raman series of ATR1XXX, ATR2XXX,ATR3XXX,ATR6XXX,ATR7XXX,ATR8XXX.
OPTOSKY provides a full series of the state of the art Raman instruments including handheld Raman, portable & desktop Raman,Raman microscope, teaching Raman, dual-band Raman, triple-band Raman, Raman PAT, high through-put Raman spectrometers. and all series can receive customization wavelength from 266-1064nm.
ATR1XXX Includes teaching Raman ATR1200 & Miniature Raman ATR1600 features a lower prices.
ATR2XXX has ATR2500 lower price uncooled portable spectrometer.
ATR3XXX Features higher performance with cooled.
ATR3000 field use with screen and built in battery,
ATR3110 lab use run with windows software.
ATR3200 dual-band Raman spectrometer.
ATR6XXX belongs to handheld Raman analyzer. It has been available in 532nm, 785nm, and 1064nm.
ATR7000 is Raman spectroscopy PAT for the Raman quantitative analysis online.532nm, 785nm, and 1064nm.
ATR8000 is All-auto & through-put Raman spectrometer can detect 100 pcs of samples per time.
ATR8XXX is the Raman microscope series including:
ATR8300 series is available single/dual-band
ATR8500 is available in single/dual/triple-band.
ATR8800 is scientific-grade Raman microscope available up to quadriband.
Q3: What is Raman Spectrometer?
A: Raman spectrometer is the scientific-class instrument used to detect molecular "fingerprint" spectrum in fast, non-contact and accurate way.
Q4: What is a Raman Spectrometer used for?
A: Raman Spectrometer is used for field analysis in fast, accurate, and non-destructive ways, It has been widely used by a non-specialist in the lab, mining, customs, law enforcement scene, pharmaceuticals, policy, metro, security safety, etc. It has been widely used for identifying materials spectrum.
Q5: What does Raman Spectrometer spectrum tell you?
A: Raman Spectrometer tells molecular vibration activities, Raman spectrum indicates material fingerprint, the real diamond has unique fingerprint spectrum, say, the Raman spectrometer can tell diamond to be detected are real or fake. The pharmaceutical drugs composition are 100% compliant.
Q6: How much does a Raman Spectrometer cost?
A: Raman spectrometer costs ranging from USD15,000 to 90,000.
Q7: How does Raman Spectrometer work?
A: Raman spectrometer software can run in the Windows or Android system, software functions include basic scan spectrum, library match, visualize microscope image on the computer, database storage and transfer, other customized functions.
Q8: How to choose an ideal Raman Spectrometer?
A: Here summarize questionair list to make a general evaluation before selection.
Q9: Where to buy Raman Spectrometer?
A: Optosky Raman Spectrometer is available in local distributors, online shop or inquire us.
Q10: Why choose Optosky Raman Spectrometer?
A：
1st, Optosky has been developing Raman spectrometer for 20-year.
2nd, Optosky is China National Standards drafter of Raman spectrometer
3rd, Top 1 in China brand and Top 3 brand in the world.
In 2016 launch the first generation handheld Raman spectrometer,
In 2017 launch the dual-band Raman spectrometer
In 2020, launch the triple-band Raman microscope
In 2021, launch the quadriband scientific-grade Raman microscope
4th, Optosky represents the highest performance cost less Chinese high-tech manufacturers with industry-leading warranty guarantee and technical support.
5th, Optosky's Raman spectroscopy technology originate from Shanghai Institute of Technical Physics Chinese Academic of Science University and Xiamen University Zhong Qun Tian who discovers SHINERS makes SERS can be preserved longer than 2 years.
6th, Quality control system follows Agilent Technologies
7th, 60% R&D engineers provide Industrial-leading warranty guarantee and technical support services
The most experienced engineers and 3 Drs team in Raman spectroscopy
8th, Complete certification of ISO, CE, FDA Approval, Police Approval, API Approval
Q11: What is the application of Raman spectrometer used in public safety?
A: When we still vividly remember the unpleasant memories happened in 2014 Xiamen city and a burned bus on which the passengers bringing the petrol along and bus burned suddenly killing many passengers. If the Raman spectrometer can be used to detect the explosives flammable and hazardous liquids on the entrance can avoid this accident. It takes few seconds to detect results, entrance and exit on the Metro, airport, BRT, bus, stadium, and large public places. And also government court, Police, Jail and fire fighting.
Q12: What is the application of Raman spectrometer used in public safety?
A: In Jail, there are some criminals have potential intention of killing themselves. These criminals could find ways to bring in drugs, toxic substance inside jail. It’s needed to detect the presence of these toxic substance before entering jail. In court, there are some defendants who threatening to kill themselves to prove their own innocent. It’s necessary to use Raman spectrometer to detect the toxic substances before entering the court. The police arrest drug dealers and few criminal investigations and illegal factory check. Only the applications related to the drug enforcement administration, customs entry and exit, and police border defenses and fire brigade and court and jail. It’s known that fentanyl has many hundreds of variance and a Raman spectroscopy is very good tool to take fast take hundreds fentanyl variants to perform field criminal investigation on illegal factory check. Such as the 1064nm handheld Raman spectrometer can export good spectra of methamphetamine, Ketamine, Ephedrine. Etc.
Q13: What is the application of handheld Raman analyzer used to identify gemstones?
A: Handheld Raman analyzer, Portable Raman spectrometer, and Raman microscope are used to identify gemstones, Jade bracelet, diamond ring. Raman spectroscopy technology is the a great tool to identify true and take gemstones.
Q14: What is the application of Raman spectrometer used in biomedical industry?
A: Raman Spectrometer is applied to biomedical industry, such as non invasive blood glucose. The Samsung company announced the non invasive blood glucose wristband applied Raman spectroscopy technology. Apple company is researching on it. These two well-known companies had already developed the finished blood glucose wristband, say, non invasive blood glucose wristband have already made the industrialization possible. And the Raman spectrum can display different blood concentrations shown on a cell phone APP.
Q15: What is the Raman spectrometer applied to detect cancer?
A: In the published paper reviewed that the patients diagnosed cancer can be identified by Raman spectroscopy. The patient diagnosed cancer shows the peak in 751nm, and the healthy person shows the peak in 758nm, there are 7cm-1 shift and and this paper writer confer the accuracy is high. In addition, the Raman spectroscopy is applied to detect skin cancer.
Q16: How is the Raman spectroscopy technology was discovered?
A: here is a very interesting story told in 1920s, an Indian scientist named Raman discover why the sea is so blue, whose blue brighter than blue sky, and after his research, he finally found in 1928 that the sea has absorbed uv range and generated neTw blue lights. Raman write a paper of his discovery, in order to remember Raman scientist great discovery and people use his name to describe this phenomenon Raman Spectroscopy. The first Discovery of Raman spectroscopy in 1928. And in 1930s, Raman scientist has won the Nobel prize. In the scientists history, Raman spectroscopy has been a very big discovery.
Q17: What is the Raman spectroscopy working principle?
A: When the molecule absorbs laser energy and generate energy level shift, but it is an unstable state. It can ring-down to the original energy level. If no energy shift part called Rayleigh scattering. However some part shift from E0 to E2, fall down to E1, This situation is called STOCKS, a single photon energy is lower energy & frequency than the laser energy but longer wavelength. The other part from E1 to higher energy level E1+hv0, and its ring down to E0 with the higher frequency and energy, but shorter wavelength called anti-stokes lines. In these situations still ring down to the deeper energy levels but less. Raman spectroscopy are mainly analyzing the STOKES lines.
Q18: How is the Raman spectrometer work?
A: First of all, the narrow linewidth laser generates an excitation light go through the fiber and collimator mirror to form a parallel incident light, excitation light focus from here, and shoot on the sample to be detected. The sample absorbs excitation light energy and it happens the energy levels shift, then go through ring-down process of generating new Raman light with a 360 degree scattering. Some portions of Raman scattering can be collected by our objective lens, then reflected from here, through long pass filter Rayleigh scattering, then couple into inside fiber and spectrometer. Raman spectrometer is used to analyze the collected Raman signal, wavelength, and intensity. Finally, it can get the Raman signal.
Q19: How is the Raman microspectrometer work?
A: The CCD export signal going through circuit, DC power, and USB, then transfer on the PC software, and finally obtain spectrum result.
Q20: What is the application of Raman spectrometer?
A:
1st, Biomedical industry of early cancer detection, ultrastructure change of biological molecule, calculi components analysis.
2nd, Public Safety of Drugs and Narcotics, Fast Detect Toxic Substance, explosives & Flammable materials, Drink water.
3rd, Environmental science of waste water contamination, petroleum analysis, deposits analysis.
4th, Pharmaceutical Engineer, Drugs scan and analysis, Crystallization in process monitor, Raw material QA & QC
5th, Food Safety, Detect refined gutter oil, Detect pesticides residues, Food additives and contaminants.
6th, Gemstones ID, Trace gemstones place-of-origin, Anti-counterfeiting, Antique ID
Q21: What are differences between Infrared spectroscopy and Raman spectroscopy?
A: Infrared spectroscopy and Raman spectroscopy are twin brothers, their spectra are mutually complementary.
1st, Working Principle Differences:
Infrared spectroscopy absorbs molecule absorption spectra, but the Raman spectroscopy absorbs the molecular emissions spectra with higher accuracy. It’s important to get a general idea that Raman spectroscopy technology is more accurate than Infrared spectroscopy.
2nd, Infrared spectroscopy is measured by contact, most infrared measurement requires sample preparation, but Raman spectroscopy requires no sample preparation and non-contact measurement through the glasses, plastic bags, kraft papers, and envelop etc.
3rd, Measure methods are different, infrared spectroscopy is destructive measurement can damage the sample requires larger samples quantities and consumable materials, but Raman spectroscopy is non-destructive measurement and can non-contact sample, no consumables materials.
4th, Infrared spectroscopy requires sample preparation, but the Raman spectroscopy requires no sample preparation.
5th,Infrared spectroscopy measure molecular group, but Raman spectroscopy measure molecular skeleton with higher accuracy.
6th,Tell the differences of isomer, including monomer isomeride, position
7th, Infrared spectroscopy can not tell the difference of isomers, but the Raman spectroscopy can tell the difference of isomers, including monomer isomer, position isomers, geometric isomers, Fentanyl variants generated in varaints fast, and Raman spectrometer has positive advantage for Fentanyl detection.
Q22: What is application of handheld Raman PHARM-ID in pharma utility?
A:
1. Raw material identification (RMID)
2. Product Development
3. Intermediate Formulation Checks
4. Brand protection / Anti-counterfeiting
5. Screening of Finished Products
Q23: What is the advantages of handheld Raman PHARM-ID rather than others?
A:
1. No sampling
2. No contact
3. Cycle time improve
4. Human resources saved for other works, efficiency improve
5. Engineer develops ID methods for new formulations
6. Wait time shorten from weeks to seconds
7. Ectronic Data Magement replace paper-driven process
Q24: Which materials have high fluorescence fit to 1064 nm Raman spectrometer?
A: Heroin, Fentanyl, Colour samples, Biological samples, Textiles, Bacteria, Drugs, Fuel, Petroleum samples, Vegetable oils, Explosives, Plastic with high fluorescence signal.
Q25: What's advantages of ATR6500 handheld Raman analyzer?
A: Compared with Thermo Fisher TruScan Raman ID, TruNarc, BWtek NanoRam, Smiths ACE-ID, Bruker , Optosky ATR6500 had made technology breakthroughs:
- Free Space Technology to shorten the scan speed to 3 seconds, super fast in the world
- The world widest wavenumber : 200-4000cm-1
- High Definition screen 1920x1080 5.5 inch touch screen
- Front & back dual camera for locked tender design and evidence pictures convenience
- The lights weight 450 g fit to one hand operate Android software with extendable memory
Q26: What's the advantages of ATR6600 and ATR6600 Pro ohandheld Raman analyzer?
A: Optosky self-designed 1064 nm handheld Raman analyzer has advantages:
- It breaks through world fastest detect speed for 1064 nm handheld Raman analyzer, say
- ATR6600 Pro Free space technology to detect in few seconds
- Wide wavenumber range and good resolution
- High definition 5.5 inch touch screen
- Lightest weight <1kg
Q27: What are differences between ATR3000-1064 vs ATR6600?
A: ATR3000-1064 has bigger size and heavier weight with better performance.
However, ATR6600 performance has reached up to the best with size, and still the most highest performance in the world for the 1064 nm handheld Raman analyzer refer to the above compare sheet.
| Items | ATR3000-1064 | ATR6600 |
| Touch screen | 11.6 inch | 5.5 inch |
| Dimension | 40x30x18 cm | 22×11×4.5 cm |
| Weight | 7.5 kg | 1.15 kg |
| Sensitivity | Better | High Performance |
| Reliability | Better | High Performance |
Q28: How much does Raman spectral Library cost?
A: Optosky free Raman spectral database including many common materials of gemstones, minerals, polymers, plastics, chemicals, narcotics, pharmaceutical drugs, and each category has several hundreds of entries attached to the Raman instrument.
Moreover, the self-building library database takes just few seconds at one button at any time in the field.
Free Library Database including but not limited to below:
1. Drugs: Heroin, Cocaine, Metamfetamine, Ketamin
2. Precursor: (-)-ephedrine, Chloroform, Diethyl ether
3. Explosives: TNT,RDX,TATP, Ammonium nitrate
4. Hazmat: sulfate, gasoline, nitric acid, Toluene
5. Food safety: Illegal food additives, residues of pesticide,veterinary drugs
5. Pharmaceutical drugs, APIs, excipients
6. Gemstones: Diamond, Agate, Emerald
7. Chemicals, Plastics, Rubber, Polymer, Compound
Q29: How To Detect Coloured samples by Handheld Raman Spectroscopy ATR6500 & ATR6600?
A: Coloured samples usually has high fluorescence, it's usually 1064 nm say ATR6600 to detect. The signal of coloured materials did not get weaker, but colour samples have the high fluorescence annihilating the Raman signals, but 1064 nm Raman spectrometer can prevent fluorescence signals from generating to annihilate Raman signals. That's why 1064 nm Raman spectrometer is developed by Optosky with the highest performance in the world.
Q30: How To Detect Solid, Liquid, Powder, or Paste type samples by Raman Spectrometer ATR6500 & ATR6600?
A: Any solid, liquid, powder, paste samples can be detected by non-contact detect.
Q31: How about materials come with plastic bag, blue drum, and carton box. Can the ATR6500 & ATR6600 Handheld Raman Analyzer detect?
A: Any transparent and semi-transparent, eg amber bottles are easy to detect through, it's needed the Raman lighting can shoot through onto the sample.
Q32: How To Detect Through Blue Drum by ATR6500 or ATR6600 Handheld Raman Analyzer?
A: The immersion probe can be mounted on the Raman probe, then dipping into the blue drum to detect the samples. refer to video:
https://optosky.com/atr6500-android1.html
Q33: How To Detect Through Opaque Container Or Carton By Handheld Raman Analyzer ATR6500 or ATR6600?
A: Open Container Or Carton To Shoot On The Samples Directly.
Q34: Is there a thickness limited that Raman spectrometer ATR6500 or ATR6600 can detect?
A: It has no thickness limit if the Raman light can shoot on the materials to be detected, you can refer to long distance and thick detect video test:
ATR6500 Handheld Raman Analyzer:
https://optosky.com/long-distance-test.html
ATR6600 1064 nm Handheld Raman Analyzer:
https://optosky.com/atr6600-penetrating-and-long-distance-video.html
Q35: What's fluorescence background in Raman spectroscopy?
How Raman signal is suppressed by fluorescence signal?
A: Fluorescence background has only one round peak without any obvious sharp peaks at all. Fluorescence intensity has millionth times stronger than that of Raman signal, say Raman signal is too weak to be easily suppressed by fluorescence background.
Q36: How to suppress fluorescence background in Raman spectroscopy? How many methods can suppress strong fluorescence background in Raman spectroscopy?
A: Fluorescence background interference is a difficult to resist. Methods to eliminate the fluorescence background include destroying the fluorescence molecules by strong light before sample preparation.
| No. | Methods | Principle | Advantage | Disadvantage |
| 1 | Baseline Correction Algorithm ATR6500 ATR3110-785,ATR3000-785 |
Baseline correction algorithm can remove fluorescence signal | Free algorithm operation | Limited Application Peaks loss Weak signal intensity Small dynamic range |
| 2 | Differential Raman Spectroscopy ATR3200 |
Fluorescence background can not shift with different excitation frequency,by using two adjacent wavelengths of lasers(eg:784.5nm+785.5nm) to excite samples can obtain two groups of signals, by differential operation obtain only Raman spectrum | Low cost of two lasers | peaks loss Low resolution Big noise Uneven shift (left high, right low) Unstable Baseline can cause shake in a mess |
| 3 | 1064nm Raman Spectrometer ATR6600-1064 ATR3000-1064 |
NIR photon can not be absorbed, so fluorescence background is not generated, in mechanism fluorescence resistance. | It resists fluorescence generation, excellent to reduce fluorescence, perfect advantages. | Higher cost 1064 nm has weak efficiency taking longer detect time (30-40s) InGaAs sensor cooled down to -20℃ with large power consumption |
| 4 | Time gated Raman Spectrometer (Cutomization) |
Raman and fluorescence scattered photons have different lifetimes. Raman photons are observed instantly during excitation, whereas fluorescence photons can still be detected after nanoseconds or even milliseconds, and thus the fluorescence background can be suppressed, if scattered photons are collected only during Raman scattering. | greater accuracy in both material identification and quantitative analysis. | Expensive time-gated laser cost |
| 5 | Photo-bleaching (532 )nm ATR3000-532 |
Due to irradiation fluorescence originating from the background decreased, whereas Raman scattering features of samples stayed unchanged. The applied photobleaching procedure satisfactorily quenched fluorescence in 90% of examined samples and made identification possible. | Material cost keep unchanged | Time consuming for continous photobleaching procedures till the fluorescence background decrease |
Q37: How to suppress fluorescence background by baseline correction algorithm?
How is fluorescence suppression result by Optosky's algorithm?
A: If Raman signal peaks can be appeared can use Optosky algorithm of baseline correction to generate elegant Raman spectrum.
But if the peaks were completed suppressed by fluorescence background, or the Raman signal is too weak to be appear can not workable by baseline correction.
Baseline correction has disadvantage of weak Raman signal and low dynamic range.
Q:38 Which materials have high fluorescence signals prefer to choose 1064 nm Raman spectrometer to suppress fluorescence?
A: Heroin, Fentanyl, colour samples , biological samples, Textiles , Bacteria , Drugs ,Fuel , Petroleum samples , Vegetable oils , Explosives , Plastics etc.
Q:39 How to choose 1064 nm Raman spectrometer models in stock?
A: Field work with built-in battery :
ATR6600 Pro , ATR6600 , ATR3000-1064 , ATR8300-1064
Laboratory with powerful software functions:
ATR3110-1064 , ATR8300-1064 , ATR8800
Q40: What's lead time for ATR8800 scientific-grade qudri-band Raman microscope?
A: It's the most advanced quadri-band Raman microscope in the world, which requires excellent craftmanship, skilled commissiong engineer with bachelor degree, it's also customized instrument, as well as long lead time high precision optical components.
Q41: How to measure black plastic sample with high heating effect?
A: An additional liquid nitrogen platform designed can resist heating effect, the articles published that with the 785 nm Raman analyzer set at a laser power of 10mw, integration time of 30 s can obtain good spectrum.
Q42:What is the NOHD (Nominal Ocular Hazard Distance) of ATR6500 in meters?
A:The following figure shows the calculation method:
Q42:What is the differece of ATR3110 and ATR3000 in drug application?
A :The application of ATR3110 and ATR3000: ATR3110 and ATR3000 can be both used for drug detection.
ATR3110 does not have a built-in battery, so it is not convenient to take it outdoors. The advantage of ATR3110 supporting PC software is that the software functions are more complete and powerful, focusing on research and analysis of materials.
The ATR3000 comes with a 10-inch tablet and built-in battery. Easy to carry outdoors, the PAD upload software can match material name, and directly match the name of the material.
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