Application of Raman Spectrometer in Various Links of Tobacco Production
author: cily
2022-01-11
The focus of competition in the tobacco trade market is tobacco quality, and the quality of tobacco is reflected by its chemical composition. Therefore, the determination of the chemical composition of tobacco plays a very important role. The research on the relationship between the chemical composition of tobacco and smoke and the quality of tobacco is mainly studied from five aspects: appearance quality, internal quality, physical characteristics, chemical composition, and safety.
Raman spectroscopy analysis is a fast, non-destructive testing technique that uses the characteristics of emission, absorption or scattering spectra of various chemical substances (including atoms, groups, molecules and polymer compounds) to determine their properties, The structure or content has been widely used at home and abroad. With the development of science and technology, the spectral resolution and instrument signal-to-noise ratio are getting higher and higher, and the accuracy of the data obtained from the spectrum is getting higher and higher, which plays an increasingly important role in the modern tobacco and food fields.
In tobacco companies, Raman spectroscopy technology has been widely used in every link (selection of raw materials, production process and process adjustment, sales process) with its own advantages.
· Application Case 1 ·
flue-cured and sun-cured tobacco
Figure 1 shows the laser Raman/fluorescence spectra of different samples. The upper tobacco leaves have the highest nicotine content and the lowest water-soluble total sugar content; the middle tobacco leaves have the lowest nicotine content and water-soluble total sugar content; Highest sugar content. We know that mature flue-cured tobacco leaves are golden or orange in color after conditioning, with high sugar content, medium nicotine content, loose tissue, rich oil content and good aroma.
Figure 2 shows the laser Raman/fluorescence spectra of two kinds of sun-cured tobacco. It can be seen from the figure that the spectral lines and peaks of SY-1 are higher than those of SY-2. After the sun-cured tobacco leaves are harvested, they are prepared under the sun. Generally, the leaves are larger, thicker, rich in oil, and the color is mostly dark yellow, purple or reddish brown. Generally, the tobacco leaves on the upper part of the plant are of better quality, with strong strength, strong smoldering firepower when burning, low sugar content in tobacco leaves, and high nicotine content.
· Application Case 2 ·
Relationship between Carotenoids in Tobacco Leaf and Tobacco Quality
The color of tobacco is a comprehensive expression of the original pigments and pigment sources in fresh tobacco leaves and the colored substances formed by the browning reaction during the brewing process. The plant pigment in fresh tobacco leaves is mainly chlorophyll, and the color of carotenoids is covered by the green color of chlorophyll. During the roasting process, chlorophyll is rapidly degraded under the action of chlorophyllase, while the degradation of carotenoids is mainly caused by oxidation, and its speed is much lower than the degradation rate of chlorophyll, resulting in a constant increase in the ratio of carotenoids to chlorophyll content. It has a comprehensive effect on the color of tobacco leaves, making the color of tobacco leaves change from green to yellow, and the appearance quality of tobacco leaves is obviously improved.
Because of its unique chain structure, carotenoids are relatively easy to be excited to generate strong Raman signals, so laser Raman spectroscopy has also been widely used in the determination of carotenoids.
In recent years, Ge Tong et al. have used Raman spectroscopy to simultaneously determine the contents of lutein and β-carotene in tobacco. The paper mentions:
Fig.3 Raman spectra of tobacco samples and standard Raman spectra of lutein, β-carotene and n-hexane
It can be observed from Figure 3 that the Raman signals of lutein and β-carotene are mainly concentrated in the two bands of 798.2~1752.8 and 2254.2~2784.5cm-1. The Raman peak at 1529cm-1 corresponds to the stretching vibration of the C=C bond, which is one of the most important characteristic peaks of the polyene chain. Another strong characteristic peak at 1159cm-1 corresponds to the stretching vibration of the C—C bond. The characteristic peak at 1006cm-1 is due to the rocking vibration between the methyl group and the carbon chain. According to the standard Raman spectra of lutein and β-carotene in Figure 5, it can be observed that there are serious peak overlaps at 1529, 1159 and 1006 cm-1 in tobacco samples.
Li Congmin et al. found that the concentration of free radicals in tar decreased with the addition of lutein and β-carotene, indicating that carotenoids have a significant scavenging effect on free radicals in cigarette tar. Based on the above effects of carotenoids in tobacco on tobacco quality, establishing a simple and effective analysis method for carotenoids has reference value for evaluating tobacco quality. The role of free radicals, reducing the impact of smoking on health also has important practical significance.
Raman spectroscopy has the characteristics of simple operation, short measurement time, and the ability to provide information about molecular functional groups. Therefore, the application of Raman spectroscopy to detect lutein and β-carotene in tobacco is a good fast detection method. The organic solvent extract of the tobacco sample was sealed in a transparent glass bottle, and the laser was focused on the solution sample in the bottle to directly measure the Raman spectrum.
Recommended Use
Raman spectrometer (532, 785nm excitation wavelength), the range is 200-3600cm-1
A new green plant detection method has been added to the ATP9100 ground object spectrometer, which can measure its chlorophyll, carotenoids and other functions, and can be used in the tobacco production process.
Raman spectroscopy analysis is a fast, non-destructive testing technique that uses the characteristics of emission, absorption or scattering spectra of various chemical substances (including atoms, groups, molecules and polymer compounds) to determine their properties, The structure or content has been widely used at home and abroad. With the development of science and technology, the spectral resolution and instrument signal-to-noise ratio are getting higher and higher, and the accuracy of the data obtained from the spectrum is getting higher and higher, which plays an increasingly important role in the modern tobacco and food fields.
In tobacco companies, Raman spectroscopy technology has been widely used in every link (selection of raw materials, production process and process adjustment, sales process) with its own advantages.
· Application Case 1 ·
flue-cured and sun-cured tobacco
Figure 1 shows the laser Raman/fluorescence spectra of different samples. The upper tobacco leaves have the highest nicotine content and the lowest water-soluble total sugar content; the middle tobacco leaves have the lowest nicotine content and water-soluble total sugar content; Highest sugar content. We know that mature flue-cured tobacco leaves are golden or orange in color after conditioning, with high sugar content, medium nicotine content, loose tissue, rich oil content and good aroma.
Figure 2 shows the laser Raman/fluorescence spectra of two kinds of sun-cured tobacco. It can be seen from the figure that the spectral lines and peaks of SY-1 are higher than those of SY-2. After the sun-cured tobacco leaves are harvested, they are prepared under the sun. Generally, the leaves are larger, thicker, rich in oil, and the color is mostly dark yellow, purple or reddish brown. Generally, the tobacco leaves on the upper part of the plant are of better quality, with strong strength, strong smoldering firepower when burning, low sugar content in tobacco leaves, and high nicotine content.
· Application Case 2 ·
Relationship between Carotenoids in Tobacco Leaf and Tobacco Quality
The color of tobacco is a comprehensive expression of the original pigments and pigment sources in fresh tobacco leaves and the colored substances formed by the browning reaction during the brewing process. The plant pigment in fresh tobacco leaves is mainly chlorophyll, and the color of carotenoids is covered by the green color of chlorophyll. During the roasting process, chlorophyll is rapidly degraded under the action of chlorophyllase, while the degradation of carotenoids is mainly caused by oxidation, and its speed is much lower than the degradation rate of chlorophyll, resulting in a constant increase in the ratio of carotenoids to chlorophyll content. It has a comprehensive effect on the color of tobacco leaves, making the color of tobacco leaves change from green to yellow, and the appearance quality of tobacco leaves is obviously improved.
Because of its unique chain structure, carotenoids are relatively easy to be excited to generate strong Raman signals, so laser Raman spectroscopy has also been widely used in the determination of carotenoids.
In recent years, Ge Tong et al. have used Raman spectroscopy to simultaneously determine the contents of lutein and β-carotene in tobacco. The paper mentions:
Fig.3 Raman spectra of tobacco samples and standard Raman spectra of lutein, β-carotene and n-hexane
It can be observed from Figure 3 that the Raman signals of lutein and β-carotene are mainly concentrated in the two bands of 798.2~1752.8 and 2254.2~2784.5cm-1. The Raman peak at 1529cm-1 corresponds to the stretching vibration of the C=C bond, which is one of the most important characteristic peaks of the polyene chain. Another strong characteristic peak at 1159cm-1 corresponds to the stretching vibration of the C—C bond. The characteristic peak at 1006cm-1 is due to the rocking vibration between the methyl group and the carbon chain. According to the standard Raman spectra of lutein and β-carotene in Figure 5, it can be observed that there are serious peak overlaps at 1529, 1159 and 1006 cm-1 in tobacco samples.
Li Congmin et al. found that the concentration of free radicals in tar decreased with the addition of lutein and β-carotene, indicating that carotenoids have a significant scavenging effect on free radicals in cigarette tar. Based on the above effects of carotenoids in tobacco on tobacco quality, establishing a simple and effective analysis method for carotenoids has reference value for evaluating tobacco quality. The role of free radicals, reducing the impact of smoking on health also has important practical significance.
Raman spectroscopy has the characteristics of simple operation, short measurement time, and the ability to provide information about molecular functional groups. Therefore, the application of Raman spectroscopy to detect lutein and β-carotene in tobacco is a good fast detection method. The organic solvent extract of the tobacco sample was sealed in a transparent glass bottle, and the laser was focused on the solution sample in the bottle to directly measure the Raman spectrum.
Recommended Use
Raman spectrometer (532, 785nm excitation wavelength), the range is 200-3600cm-1
A new green plant detection method has been added to the ATP9100 ground object spectrometer, which can measure its chlorophyll, carotenoids and other functions, and can be used in the tobacco production process.
Spectral non-destructive testing technolog 
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