Product Recommendation: UV3000 UV-Vis Spectrophotometer

2025-01-24

Overview

Optosky’s UV3000 features advanced optical design for precise results. Equipped with a dual-beam detection system, it ensures exceptional accuracy and reliability.

Operating Principle

The spectrophotometer determines substance concentration and structure by measuring light absorption in the UV-Vis spectrum. The light source emits composite light, which is decomposed into monochromatic light by the monochromator. After passing through the sample, the detector converts the transmitted light into electrical signals, providing absorbance data. The absorption spectrum reflects molecular and atomic interactions at specific wavelengths, involving vibrational and electronic energy level transitions.

Components

Light Source: Supplies stable, high-intensity continuous spectra. The UV3000 offers deuterium-tungsten lamps or pulsed xenon lamps. The pulsed xenon lamp eliminates preheating, saves time, and delivers long-term performance with minimal maintenance.
Monochromator: Separates and selects the desired wavelength of light. The UV3000 series employs grating monochromators for high resolution, broad wavelength range, and uniformity.
Sample Cell: Holds the sample for absorbance measurement. Quartz cells are used for UV-Vis regions, while glass cells are for visible light.
Detector: Converts light signals into electrical signals. Options include phototubes, photomultiplier tubes, and photodiode arrays.
Display: Amplifies and visualizes data. UV3000 features USB connectivity for PC data processing. The UV3000TP model includes a 10-inch HD touchscreen for standalone operation and visualized results. Indicator lights provide intuitive status updates.

Advantages

High Sensitivity: Detects low-concentration samples.
Superior Accuracy: Ultra-low stray light ensures precise concentration and purity analysis.
User-Friendly: Simple setup, straightforward operation, and fast analysis.
High Reproducibility: Delivers consistent results under identical conditions.
Versatility: Applicable for compound identification, purity testing, reaction kinetics, and unknown concentration determination.
Efficiency: Automated 6-channel cuvette holder accelerates sample processing.

Applications

Environmental Monitoring: Detects pollutants in water, soil, and air.

Fig.1 UV absorption spectra of TOC samples indicate organic carbon concentration at 245 nm.

TOC samples show significant absorption of light in the wavelength range of 200–310 nm, with the highest absorption peak around 245 nm. The organic carbon content in water can be estimated based on the absorbance intensity.

Chemical Analysis: Determines components in pharmaceuticals, cosmetics, and food.

Figure 2. UV-Vis Spectra and PLS-DA Classification Results of Licorice from Different Varieties and Origins

(a) UV-Vis spectra of extracts from different licorice varieties;

(b) UV-Vis spectra of extracts from licorice of different origins;

(d) PLS-DA classification results for origins.

The glycyrrhizin content in licorice is influenced by factors such as soil, growth environment, planting time, variety, storage, processing, and cultivation techniques, resulting in variations among different varieties and origins. As shown in Figures 1(a, b), the UV-Vis spectra of licorice differ across varieties and origins, indicating that UV-Vis spectroscopy is effective in distinguishing licorice varieties and origins.

Agriculture & Aquaculture: Measures pesticide residues, crop composition, and veterinary drugs.

Figure 3. PCA Inhibition Effect on CD Production

Figure 3 shows the process of CD formation during LDL oxidation. Early in the oxidation process, endogenous antioxidants in LDL (such as vitamin E) protect it from oxidative modification. As lipid oxidation progresses, the endogenous antioxidants are consumed, and CD formation increases sharply. The oxidation delay time is shortest without PCA, but after adding PCA at different concentrations, the delay time is significantly extended.

Product Line Specifications

Model	UV3000A	UV3000B	UV3000C	UV3000D
Wavelength Range	180~1100nm
Optical System	Dual-beam
Sample Channels	6 channels, automatic switching
Wavelength Accuracy	±0.3 nm
Wavelength Repeatability	≤0.1 nm
Bandwidth	0.5nm、1.0nm、2.0nm、4.0nm
Transmittance repeatability	±0.15%
Stray Light	≤0.03%
Measurement Modes	Transmittance, Absorbance, Concentration, Energy
Wavelength adjustment	Automatic
Luminosity range	-4~4A
Light Source	Long-life pulsed xenon lamp	Long-life pulsed xenon lamp	Deuterium-halogen combination lamp	Deuterium-halogen combination lamp
Detector	Photocell	Photomultiplier tube	Photocell	Photomultiplier tube
Noise Level	±0.001Abs	±0.0005Abs	±0.001Abs	±0.0005Abs
Photometric accuracy	±0.3%T	±0.3%T	±0.2%T	±0.2%T
Output	USB Interface
Dimension	455 × 286 × 225mm
Power Supply	AC220V，100W

Note：

Wavelength range can be customized.
The optimal resolution is related to the width of the spectrometer’s slit. Further narrowing the slit can enhance the resolution.
The parameters in the table represent those of the standard products. All Optosky instruments are independently developed and manufactured, and corresponding parameters can be customized.