Measuring Solution Absorbance Comparison by UV-VIS Spectrometer
author: Susan
2022-01-04
Traditional chemical methods have high water quality detection accuracy, but the detection cycle is long, requires professionals to operate, and the chemical reagents used are prone to secondary pollution. In recent years, ultraviolet-visible spectroscopy has been widely used in water quality testing. It has the advantages of fast speed, no secondary staining, fingerprint detection, and traceability of pollution. It has become a research hotspot in the field of water quality testing. In the UV-visible spectroscopy for the water quality detection system, in order to improve the accuracy and stability of the entire detection system, higher requirements are placed on the data spectrum detection unit.
In this experiment, different concentrations of Potassium Hydrogen Phthalate Solution were used to conduct comparative test experiments on the Hamamatsu C10082CAH spectrometer, the Ocean Insight Maya2000Pro spectrometer, and Optosky ATP2000P spectrometer.
Principle: Lambert-Beer Law is the basic law of aw of absorption spectroscopy. When a beam of parallel monochromatic light passes through a uniform, non-scattering light-absorbing solution vertically, its absorbance A is proportional to the concentration of the light-absorbing solution c and the thickness of the liquid layer (optical path) l. This law is also quantitative by ultraviolet spectroscopy. The theoretical basis of the analysis, the schematic diagram of the Lambert-Beer law is shown in Figure 1, which can be expressed as
Figure 1 Schematic diagram of Lambert-Beer law
The eight groups of sample solutions used in the experiment are all Potassium Hydrogen Phthalate solutions of different concentrations prepared by chemical methods, and used as standard concentrations of chemical
Oxygen demand (COD) solutions for experimental measurement. The concentrations are respectively 20, 40, 60, 120, 160, 200, 400 and 800 mg·L-1.
Spectrum of 8 standard sample solution measured under these spectrometers below figure1, 2 and 3:
Figure 3 Hamamatsu C10082CAH spectrometer
Figure 4 Ocean Insight Maya2000 Pro spectrometer
Figure 5 Optosky ATP2000P spectrometer
In this experiment, different concentrations of Potassium Hydrogen Phthalate Solution were used to conduct comparative test experiments on the Hamamatsu C10082CAH spectrometer, the Ocean Insight Maya2000Pro spectrometer, and Optosky ATP2000P spectrometer.
Principle: Lambert-Beer Law is the basic law of aw of absorption spectroscopy. When a beam of parallel monochromatic light passes through a uniform, non-scattering light-absorbing solution vertically, its absorbance A is proportional to the concentration of the light-absorbing solution c and the thickness of the liquid layer (optical path) l. This law is also quantitative by ultraviolet spectroscopy. The theoretical basis of the analysis, the schematic diagram of the Lambert-Beer law is shown in Figure 1, which can be expressed as
Figure 1 Schematic diagram of Lambert-Beer law
Spectroscopy Solution:
In the experiment, three typical spectrometers used in the water quality spectrum detection were used to detect samples, namely: Hamamatsu C10082CAH spectrometer, Ocean Maya2000Pro spectrometer, and Optosky ATP2000P spectrometer, among which Hamamatsu C10082CAH spectrometer from 200~800nm, measuring 2048 pixels; Ocean Insight Maya2000Pro spectrometer from 200~1100nm, measuring 2068 pixels; Optosky ATP2000P spectrometer, measuring 2048 pixels from 200 to 1,100 nm. The water quality detection system is shown in Figure 2. In order to verify the validity of the experimental comparison, the experiment is the same except for the spectrometer, the other experimental environment and test equipment are the same: the same light source ATG1020H, the same optical path ATP0080, and the optical fiber UV-VIS.
In the experiment, three typical spectrometers used in the water quality spectrum detection were used to detect samples, namely: Hamamatsu C10082CAH spectrometer, Ocean Maya2000Pro spectrometer, and Optosky ATP2000P spectrometer, among which Hamamatsu C10082CAH spectrometer from 200~800nm, measuring 2048 pixels; Ocean Insight Maya2000Pro spectrometer from 200~1100nm, measuring 2068 pixels; Optosky ATP2000P spectrometer, measuring 2048 pixels from 200 to 1,100 nm. The water quality detection system is shown in Figure 2. In order to verify the validity of the experimental comparison, the experiment is the same except for the spectrometer, the other experimental environment and test equipment are the same: the same light source ATG1020H, the same optical path ATP0080, and the optical fiber UV-VIS.
Figure 2 UV-Visible spectroscopy in water quality detection system
Test Result:The eight groups of sample solutions used in the experiment are all Potassium Hydrogen Phthalate solutions of different concentrations prepared by chemical methods, and used as standard concentrations of chemical
Oxygen demand (COD) solutions for experimental measurement. The concentrations are respectively 20, 40, 60, 120, 160, 200, 400 and 800 mg·L-1.
Spectrum of 8 standard sample solution measured under these spectrometers below figure1, 2 and 3:
- Figure 1: In the range of 20~800 mg·L-1, the absorbance range of the first characteristic peak is 2.4 ~ 3.25, the wavelength range is a 230 ~ 255 nm, and the absorbance range of the second characteristic peak is 0.4 ~ 3.1 near 280nm.
- Figure 2: In the range of 20~800 mg·L-1, the absorbance of the first characteristic peak is in the range of 1.3 ~ 2.2 nm, and the wavelength range is a 235 ~ 260 nm, the absorbance of the second characteristic peak is in the range of 0.2 ~ 2, and the wavelength range is a 270 ~ 300 nm.
- Figure 3: In the range of 20~800 mg·L-1, the absorbance of the first characteristic peak is in the range of 1.3 ~ 2.4, and the wavelength range is a 230 ~ 255 nm, the absorbance of the second characteristic peak is in the range of 0.3 ~ 2.3, and the wavelength range is a 275 ~ 300 nm.
Figure 3 Hamamatsu C10082CAH spectrometer
Figure 4 Ocean Insight Maya2000 Pro spectrometer
Figure 5 Optosky ATP2000P spectrometer
Conclusion:
Through many experiments and comparisons, for the UV range of 230-300nm, Optosky's ATP2000P spectrometer has the closest wavelength accuracy to Hamamatsu's C10082CAH spectrometer. At the same time, the characteristic peak absorbance is the same as that of the Ocean Insight's Maya2000Pro, even better than Maya2000Pro.
It can be seen that ATP2000P is a very cost-effective spectrometer that is very suitable for detecting water quality, and the price is only one-eighth of Maya2000Pro.
Related Products:
ATP2000P Deuterium Halogen Light source ATG1020H Cuvette Holder ATP0080 Optical fiber UV-VIS
Related Articles:
Through many experiments and comparisons, for the UV range of 230-300nm, Optosky's ATP2000P spectrometer has the closest wavelength accuracy to Hamamatsu's C10082CAH spectrometer. At the same time, the characteristic peak absorbance is the same as that of the Ocean Insight's Maya2000Pro, even better than Maya2000Pro.
It can be seen that ATP2000P is a very cost-effective spectrometer that is very suitable for detecting water quality, and the price is only one-eighth of Maya2000Pro.
Related Products:
ATP2000P Deuterium Halogen Light source ATG1020H Cuvette Holder ATP0080 Optical fiber UV-VIS
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- “Water, Air & Soil Pollution,”
- “Spectroscopy and Spectroscopic Analysis”
- “Online Monitoring of Water-Quality Anomaly in Water Distribution Systems Based on Probabilistic Principal Component Analysis by UV-Vis Absorption Spectroscopy”
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